pTXB1-His6-IntCtr-GyrA-His6
(Plasmid
#126019)
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PurposeBacterial expression of IntCtr-GyrA fusion protein (with N- and C-terminal His6)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTXB1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHis6-IntCtr-GyrA-His6
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- GyrA-6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTXB1-His6-IntCtr-GyrA-His6 was a gift from Thomas Muir (Addgene plasmid # 126019 ; http://n2t.net/addgene:126019 ; RRID:Addgene_126019)
