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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET23
- Backbone size (bp) 4300
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Modifications to backboneF271C, H246,249D
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCdc42 Biosensor Control
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Alt nameCBD(201-314)-F271C-H246,249D-EGFP
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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Tags
/ Fusion Proteins
- EGFP (C terminal on insert)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!
Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET23-CBD-(-PP)-EGFP-H246,249D was a gift from Klaus Hahn (Addgene plasmid # 12598 ; http://n2t.net/addgene:12598 ; RRID:Addgene_12598) -
For your References section:
Activation of endogenous Cdc42 visualized in living cells. Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM. Science. 2004 Sep 10. 305(5690):1615-9. 10.1126/science.1100367 PubMed 15361624
Map uploaded by the depositor.