pET23-CBD-(-PP)-EGFP
(Plasmid #12597)

• Depositing Lab: Klaus Hahn
• Publication: Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Backbone

• Vector backbone: pET23
• Backbone size w/o insert (bp): 4300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cdc42 Biosensor
• Alt name: CBD(201-314)-F271C-EGFP
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: F271C
• Entrez Gene: WAS (a.k.a. IMD2, SCNX, THC, THC1, WASP, WASPA)
• Tags / Fusion Proteins:
  • EGFP (C terminal on insert)
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protocols for production and use of Hahn lab biosensors and photactivatable proteins
can be found on their web page, at the URL below. If you have
suggestions for the protocols or have any questions, please feel free to contact the Hahn
lab. Good luck with your experiments!

Hahn lab biosensor protocol: http://www.hahnlab.com/tools/index.html