VII_pAAV-ProB8-GFP-WPRE
(Plasmid
#125928)
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PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retina
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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Alt nameChR2(L132C)-EGFP
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SpeciesSynthetic
- Promoter ProB8
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/10/04/434720.full.pdf for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VII_pAAV-ProB8-GFP-WPRE was a gift from Botond Roska (Addgene plasmid # 125928 ; http://n2t.net/addgene:125928 ; RRID:Addgene_125928) -
For your References section:
Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates and humans. Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B. Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 10.1038/s41593-019-0431-2. 10.1038/s41593-019-0431-2 PubMed 31285614