pBabe HAII RLF CAAX
(Plasmid
#12592)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe HAII
- Backbone size w/o insert (bp) 5137
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRLF-CAAX
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Alt nameRLF
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SpeciesM. musculus (mouse)
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Insert Size (bp)2472
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Entrez GeneRgl2 (a.k.a. KE1.5, Rab2l, Rgt2, Rlf)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI? See author map (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe HAII RLF CAAX was a gift from Channing Der (Addgene plasmid # 12592 ; http://n2t.net/addgene:12592 ; RRID:Addgene_12592) -
For your References section:
Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K, Der CJ. Mol Cell Biol. 2001 Aug . 21(16):5488-99. 10.1128/MCB.21.16.5488-5499.2001 PubMed 11463831
Map uploaded by the depositor.