Skip to main content
Addgene

pEGFP-SUN1_916
(Plasmid #125850)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125850 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    ｐEGFP-N3
  • Backbone manufacturer
    clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SUN1_916
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2748
  • Mutation
    stop codon
  • GenBank ID
    EAW87177
  • Entrez Gene
    SUN1 (a.k.a. UNC84A)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Sal I (unknown if destroyed)
  • 5′ sequencing primer AAATTAGATCTGCCACCATGGATTTTTCTCGGCTTCACATGTACAGT
  • 3′ sequencing primer AAATTGTCGACCTTGACAGGTTCGCCATGAACTCTGAACCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-SUN1_916 was a gift from Miki Hieda (Addgene plasmid # 125850 ; http://n2t.net/addgene:125850 ; RRID:Addgene_125850)

  • For your References section:

    SUN1 splice variants, SUN1_888, SUN1_785, and predominant SUN1_916, variably function in directional cell migration. Nishioka Y, Imaizumi H, Imada J, Katahira J, Matsuura N, Hieda M. Nucleus. 2016 Nov;7(6):572-584. doi: 10.1080/19491034.2016.1260802. 10.1080/19491034.2016.1260802 PubMed 27858498
Related items:
Commonly requested with: