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RCAS-Neu HA SIINFEKL
(Plasmid #125832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCAS-Y
  • Backbone manufacturer
    Yi Li
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Neu
  • Alt name
    erb-b2 receptor tyrosine kinase 2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1400
  • Mutation
    Val to Glu point mutation of codon 664 and truncations at extracellular and intracellular domains
  • Entrez Gene
    Erbb2 (a.k.a. HER2/neu, neu)
  • Tags / Fusion Proteins
    • 2x HA (C terminal on insert)
    • SIINFEKL (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS-Neu HA SIINFEKL was a gift from Yi Li (Addgene plasmid # 125832 ; http://n2t.net/addgene:125832 ; RRID:Addgene_125832)

  • For your References section:

    Introduction of oncogenes into mammary glands in vivo with an avian retroviral vector initiates and promotes carcinogenesis in mouse models. Du Z, Podsypanina K, Huang S, McGrath A, Toneff MJ, Bogoslovskaia E, Zhang X, Moraes RC, Fluck M, Allred DC, Lewis MT, Varmus HE, Li Y. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17396-401. doi: 10.1073/pnas.0608607103. Epub 2006 Nov 7. 10.1073/pnas.0608607103 PubMed 17090666
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