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pET151-GST-PP-RPN13 (19-132) R104E
(Plasmid #125828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET151-D-TOPO
  • Modifications to backbone
    N-terminal His-tag removed
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPN13
  • Alt name
    ADRM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    339
  • Mutation
    changed arginine 104 to glutamate
  • GenBank ID
    NM_175573
  • Entrez Gene
    ADRM1 (a.k.a. ARM-1, ARM1, GP110, PSMD16)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET151-GST-PP-RPN13 (19-132) R104E was a gift from Chris Hill (Addgene plasmid # 125828 ; http://n2t.net/addgene:125828 ; RRID:Addgene_125828)

  • For your References section:

    Phosphorylation of Tyr950 in the proteasome scaffolding protein RPN2 modulates its interaction with the ubiquitin receptor RPN13. Hemmis CW, Heard SC, Hill CP. J Biol Chem. 2019 May 7. pii: AC119.008881. doi: 10.1074/jbc.AC119.008881. 10.1074/jbc.AC119.008881 PubMed 31064842
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