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PurposeMonomeric TagBFP2 and iCre recombinase (version 2)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2-T2A-iCre
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bymTagBFP2 is from Michael Davidson. iCre is from Rolf Sprengel.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An extra Valine in plasmid pCAGGS-mTagBFP2-T2A-iCre (#113837) is deleted. In the NGS sequence, polymorphisms are present in a GC-rich region of the Chicken beta-actin promoter that do not affect the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-mTagBFP2-T2A-iCre (v2) was a gift from Mario Capecchi (Addgene plasmid # 125822 ; http://n2t.net/addgene:125822 ; RRID:Addgene_125822)