pcDNA3.1-CD40L (lose of function mutant)-mgSrtA
(Plasmid
#125796)
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Purposefor co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125796 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD40L (lose of function mutant)-mgSrtA
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-CD40L (lose of function mutant)-mgSrtA was a gift from P. Chen (Addgene plasmid # 125796 ; http://n2t.net/addgene:125796 ; RRID:Addgene_125796) -
For your References section:
Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions. Ge Y, Chen L, Liu S, Zhao J, Zhang H, Chen PR. J Am Chem Soc. 2019 Feb 6;141(5):1833-1837. doi: 10.1021/jacs.8b10286. Epub 2019 Jan 29. 10.1021/jacs.8b10286 PubMed 30676735