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PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDisplay
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemgSrtA
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The Neomycin/Kanamycin (NeoR/KanR) selection marker contains a coding difference compared to the NCBI reference sequence the functional consequence of which, is unknown. The plasmid is functional for transient transfection but NeoR/KanR resistance should be functionally characterized before attempting use in generating stable cell lines or under selection.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDisplay-mgSrtA was a gift from P. Chen (Addgene plasmid # 125792 ; http://n2t.net/addgene:125792 ; RRID:Addgene_125792) -
For your References section:
Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions. Ge Y, Chen L, Liu S, Zhao J, Zhang H, Chen PR. J Am Chem Soc. 2019 Feb 6;141(5):1833-1837. doi: 10.1021/jacs.8b10286. Epub 2019 Jan 29. 10.1021/jacs.8b10286 PubMed 30676735