pUC18/promEco/Lysostaphin
(Plasmid #125765)

• Purpose: Production of staphylolytic protein lysostaphin from a constitutive promoter
• Depositing Lab: Benedykt Wladyka
• Publication: Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782. doi: 10.1007/s12033-019-00203-4.

Backbone

• Vector backbone: pUC18
• Backbone size w/o insert (bp): 2686
• Total vector size (bp): 3937
• Modifications to backbone: pemIK-Sa1 constitutive promoter added between EcoRI and SacI restriction sites
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lysostaphin
• Alt name: lss
• Alt name: end
• Species: Staphylococcus simulans biovar staphylolyticus
• Insert Size (bp): 750
• Mutation: sequence corresponding to the mature form protein
• GenBank ID: M15686.1
• Promoter: pemIK-Sa1 promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: CAGGAAACAGCTATGAC
• 3′ sequencing primer: TGTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pUC18_promEco_Lysostaphin plasmid.gbk
• A portion of this plasmid was derived from a plasmid made by: Piotr Szweda, Department of Food Chemistry, Technology and Biotechnology, Gdansk University of Technology Szweda, P., Kotłowski, R., & Kur, J. (2005). New effective sources of the Staphylococcus simulans lysostaphin. Journal of Biotechnology, 117(2), 203–213.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry