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Addgene

Destination Vector
(Plasmid #125754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK001
  • Backbone manufacturer
    John Dueber
  • Backbone size w/o insert (bp) 1662
  • Total vector size (bp) 3635
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTDH3-GFPdropout-tTDH1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1973

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Destination Vector was a gift from Rodrigo Ledesma-Amaro (Addgene plasmid # 125754 ; http://n2t.net/addgene:125754 ; RRID:Addgene_125754)

  • For your References section:

    Rapid Assembly of gRNA Arrays via Modular Cloning in Yeast. McCarty NS, Shaw WM, Ellis T, Ledesma-Amaro R. ACS Synth Biol. 2019 Apr 2. doi: 10.1021/acssynbio.9b00041. 10.1021/acssynbio.9b00041 PubMed 30939239
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