Human Interferon-Stimulated Gene CRISPR Knockout Library
(Pooled Library #125753)

• Purpose:

  This CRISPR knockout pooled library targets a set of human interferon-stimulated genes (ISGs). There are eight guides per target gene, primarily selected from existing libraries (Brunello, GeCKOv2, TKO Version 1, and Human Activity-Optimized CRISPR Knockout Library).

• Vector Backbone:

  lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

• Depositing Labs: Michael Emerman and Molly OhAinle
• Publication: Roesch et al. Retrovirology. 2018 Mar 20;15(1):26. doi: 10.1186/s12977-018-0409-2.
  OhAinle et al. eLife. 2018 Dec 6; 7: e39823. doi: 10.7554/eLife.39823

Library Details

• Species: Human
• Genes targeted: 1,902
• gRNAs: 15,416
• Controls: 200
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

• Volume: ∼20 µL
• Concentration: 50 ng/µL

Resource Information

• Protocol:
  • Primer Design and PCR Protocol (DOCX, 20 KB)
• Depositor Data:
  • Human Interferon-Stimulated Gene Library Target Genes (XLSX, 162 KB)
  • Guide RNA Sequences (XLSX, 522 KB)
  • Library Representation Histogram (PDF, 19 KB)



See our Pooled Library Amplification Protocol for Addgene's generalized library amplification protocol.

Articles Citing this Pooled Library:

• lentiCRISPRv2_ISG KO (#125753)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  Industry Terms:
  • Not Available to Industry
  Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This Human Interferon-Stimulated Gene library was developed in collaboration with the Overbaugh and Paddison labs at Fred Hutch.

This ISG-targeting CRISPR KO library was used in Roesch et al. (2018) to screen for ISGs that inhibit VSV/G-pseudotyped lentiviral particles. The curation of the list of ISGs and the library design and synthesis are described in Ohainle et al. (2018). The same pooled sgRNAs have also been cloned into an HIV-specific CRISPR knockout plasmid, the HIV-CRISPR vector, to create an HIV-specific CRISPR KO library, called the PIKA-HIV (Packageable ISG Knockout Assembly). This CRISPR KO library specific for HIV research is available through the NIH AIDS Reagent Program (Catalog #13566).

The depositing lab recommends using MAGeCK algorithm for analysis.