Guide-Generating Vector
(Plasmid
#125752)
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PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYTK001
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Backbone manufacturerJohn Dueber
- Backbone size w/o insert (bp) 1638
- Total vector size (bp) 2764
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCys4-GFPdropout-SpCas9scaffold
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Guide-Generating Vector was a gift from Rodrigo Ledesma-Amaro (Addgene plasmid # 125752 ; http://n2t.net/addgene:125752 ; RRID:Addgene_125752) -
For your References section:
Rapid Assembly of gRNA Arrays via Modular Cloning in Yeast. McCarty NS, Shaw WM, Ellis T, Ledesma-Amaro R. ACS Synth Biol. 2019 Apr 2. doi: 10.1021/acssynbio.9b00041. 10.1021/acssynbio.9b00041 PubMed 30939239
