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Addgene

pMSCV-TAP mLST8 (aka: GbL)
(Plasmid #12575)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-TAP
  • Backbone manufacturer
    Sabatini Lab (Addgene Plasmid #12570)
  • Backbone size w/o insert (bp) 6800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mLST8
  • Alt name
    GbL
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    981
  • GenBank ID
    NP_071767
  • Entrez Gene
    MLST8 (a.k.a. GBL, GbetaL, LST8, POP3, WAT1)
  • Tag / Fusion Protein
    • TAP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer MSCV rev primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMSCV-TAP was cut with XhoI/NotI and ligated to in-frame SalI/NotI inserts containing the sequences of mLST8.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-TAP mLST8 (aka: GbL) was a gift from David Sabatini (Addgene plasmid # 12575 ; http://n2t.net/addgene:12575 ; RRID:Addgene_12575)

  • For your References section:

    mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458
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