pCAG-sfGFP
(Plasmid #125747)

• Purpose: sfGFP without iCreERT2 fusion
• Depositing Lab: Mario Capecchi
• Publication: Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.

Backbone

• Vector backbone: Custom
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sfGFP
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • CAG sfGFP.gb
• A portion of this plasmid was derived from a plasmid made by: CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland. Backbone is from Stratagene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.

Nevertheless, for sfGFP expression, the depositor recommends the updated versions of this plasmid, PB CAG-sfGFP (https://www.addgene.org/133568/) or PB-CA sfGFP (https://www.addgene.org/159094/) that can also be used without the piggyBac transposase.

Red fluorescent protein versions are also available: PB CAG-tdTomato (https://www.addgene.org/133569/) or PB-CA tdTomato (https://www.addgene.org/159095/)