pB6-ROSA26-DTA-Capecchi-rev
(Plasmid #125744)

• Purpose: (Empty Backbone) B6J ROSA26 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, reverse cloning sites, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Mouse safe harbor locus targeting plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Resource Information

• Supplemental Documents:
  • B6J ROSA26 homology arms DTA, low copy number, Capecchi config, reverse linker, I-CeuI.gb
• A portion of this plasmid was derived from a plasmid made by: DTA negative selection cassette is from Philippe Soriano. p15A backbone is from Sen Wu, Capecchi laboratory.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homology arms subcloned from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

AscI and AsiSI (PacI-compatible) cloning sites in the XbaI site of ROSA26 (Soriano, 1999), a highly recombinogenic locus.

The 5' arm appears to have SNP's that differ between 129 and B6 genomes. Please see pROSA26-1 (#21714) and the reference B6J genome.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.