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Addgene

pcDNA3 Lis1
(Plasmid #12574)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12574 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3+
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lis1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1231
  • Entrez Gene
    Pafah1b1 (a.k.a. LIS-1, Lis1, MMS10-U, Mdsh, Ms10u, Pafaha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Lis1 was a gift from Li-Huei Tsai (Addgene plasmid # 12574 ; http://n2t.net/addgene:12574 ; RRID:Addgene_12574)

  • For your References section:

    Ndel1 operates in a common pathway with LIS1 and cytoplasmic dynein to regulate cortical neuronal positioning. Shu T, Ayala R, Nguyen MD, Xie Z, Gleeson JG, Tsai LH. Neuron. 2004 Oct 14. 44(2):263-77. 10.1016/j.neuron.2004.09.030 PubMed 15473966
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