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Addgene

pJin-264
(Plasmid #125717)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125717 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
  • gRNA/shRNA sequence
    unknown
  • Species
    H. sapiens (human)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PYL/ABI genes were cloned from Prof. Liang's vector. ( ref. Liang, F. S., Ho, W. Q., and Crabtree, G. R. (2011) Engineering the ABA plant stress pathway for regulation of induced proximity. Sci. Signaling 4, rs2.)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJin-264 was a gift from Bryan Dickinson (Addgene plasmid # 125717 ; http://n2t.net/addgene:125717 ; RRID:Addgene_125717)
  • For your References section:

    Multidimensional Control of Cas9 by Evolved RNA Polymerase-Based Biosensors. Pu J, Kentala K, Dickinson BC. ACS Chem Biol. 2018 Feb 16;13(2):431-437. doi: 10.1021/acschembio.7b00532. Epub 2017 Aug 22. 10.1021/acschembio.7b00532 PubMed 28809467