pJin-239
(Plasmid
#125716)
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PurposeABA-inducible split T7 gRNA for GFP vector using RNAPN N-29-1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (N-29-1)-linker-ABI
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gRNA/shRNA sequenceunknown
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPYL/ABI genes were cloned from Prof. Liang's vector. ( ref. Liang, F. S., Ho, W. Q., and Crabtree, G. R. (2011) Engineering the ABA plant stress pathway for regulation of induced proximity. Sci. Signaling 4, rs2.)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJin-239 was a gift from Bryan Dickinson (Addgene plasmid # 125716 ; http://n2t.net/addgene:125716 ; RRID:Addgene_125716) -
For your References section:
Multidimensional Control of Cas9 by Evolved RNA Polymerase-Based Biosensors. Pu J, Kentala K, Dickinson BC. ACS Chem Biol. 2018 Feb 16;13(2):431-437. doi: 10.1021/acschembio.7b00532. Epub 2017 Aug 22. 10.1021/acschembio.7b00532 PubMed 28809467