pRK5-Gqi9
(Plasmid
#125711)
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PurposeEukaryotic expression of the chimeric G protein Gqi9
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125711 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4754
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namechimericG protein Gqi9
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Alt nameGqi9
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SpeciesH. sapiens (human)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-Gqi9 was a gift from Kai Johnsson (Addgene plasmid # 125711 ; http://n2t.net/addgene:125711 ; RRID:Addgene_125711) -
For your References section:
A fluorescent sensor for GABA and synthetic GABA(B) receptor ligands. Masharina A, Reymond L, Maurel D, Umezawa K, Johnsson K. J Am Chem Soc. 2012 Nov 21;134(46):19026-34. doi: 10.1021/ja306320s. Epub 2012 Nov 5. 10.1021/ja306320s PubMed 23095089