pRK5-SNAP-CLIP-GB1a
(Plasmid
#125709)
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PurposeEukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4754
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNAP-CLIP-GB1a
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-SNAP-CLIP-GB1a was a gift from Kai Johnsson (Addgene plasmid # 125709 ; http://n2t.net/addgene:125709 ; RRID:Addgene_125709) -
For your References section:
A fluorescent sensor for GABA and synthetic GABA(B) receptor ligands. Masharina A, Reymond L, Maurel D, Umezawa K, Johnsson K. J Am Chem Soc. 2012 Nov 21;134(46):19026-34. doi: 10.1021/ja306320s. Epub 2012 Nov 5. 10.1021/ja306320s PubMed 23095089
