pT2-bactin:QF2,crybb:CFP
(Plasmid
#125697)
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PurposeExpresses QF2 ubiquitously in zebrafish, with selectable marker CFP in eye lens
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestTol2pA3
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Vector typezebrafish expression
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Selectable markersCFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebactin:QF2
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SpeciesD. rerio (zebrafish); Neurospora crassa
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2-bactin:QF2,crybb:CFP was a gift from Daniel Gorelick (Addgene plasmid # 125697 ; http://n2t.net/addgene:125697 ; RRID:Addgene_125697)