pT2-5xERE:GFP
(Plasmid
#125690)
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PurposeTransgene to report estrogen receptor activity in zebrafish
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 125690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTol2
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Vector typeZebrafish expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5xERE:GFP
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SpeciesSynthetic
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT2-5xERE:GFP was a gift from Daniel Gorelick & Marnie Halpern (Addgene plasmid # 125690 ; http://n2t.net/addgene:125690 ; RRID:Addgene_125690) -
For your References section:
Visualization of estrogen receptor transcriptional activation in zebrafish. Gorelick DA, Halpern ME. Endocrinology. 2011 Jul;152(7):2690-703. doi: 10.1210/en.2010-1257. Epub 2011 May 3. 10.1210/en.2010-1257 PubMed 21540282
