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pDEST12.2 DYRK2 KD
(Plasmid #12569)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12569 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEST12.2
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DYRK2
  • Species
    H. sapiens (human)
  • Mutation
    Kinase dead: K251R
  • Entrez Gene
    DYRK2
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kinase-dead DYRK2 was constructed by introducing a K251R point mutation in the ATP binding
pocket of the DYRK2 active site. See the WT version (Addgene plasimd # 20005) for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST12.2 DYRK2 KD was a gift from Anjana Rao (Addgene plasmid # 12569)

  • For your References section:

    A genome-wide Drosophila RNAi screen identifies DYRK-family kinases as regulators of NFAT. Gwack Y, Sharma S, Nardone J, Tanasa B, Iuga A, Srikanth S, Okamura H, Bolton D, Feske S, Hogan PG, Rao A. Nature. 2006 Jun 1. 441(7093):646-50. 10.1038/nature04631 PubMed 16511445
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