pGART-2XStrep
(Plasmid #125681)

• Purpose: expresses human GART protein with a C-terminal 2XStrep affinity tag
• Depositing Lab: Stephen Benkovic
• Publication: Liu et al J Proteome Res. 2019 May 3;18(5):2078-2087. doi: 10.1021/acs.jproteome.8b00969. Epub 2019 Apr 18.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 8568
• Modifications to backbone: addition of 2X Strep-tag II by restriction cloning at the end of the MCS
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GART
• Species: H. sapiens (human)
• Insert Size (bp): 3030
• Entrez Gene: GART (a.k.a. AIRS, GARS, GARTF, PAIS, PGFT, PRGS)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2X Strep-Tag II (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GAC ATC CAC TTT GCC TTT CT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's quality control result found a 12bp deletion in the Neomycin Resistance cassette. The depositing lab has noted that this deletion will NOT affect plasmid function as described in their publication, but they have not tested for Neomycin selection directly. We suggest testing Neomycin selection if you need to generate stable cell lines.