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UBQ10:sXVE:ER-GFP1–10
(Plasmid #125664)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    UBQ10:sXVE:(MCS)-S11
  • Backbone manufacturer
    Tzu-Yin Liu
  • Total vector size (bp) 15101
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ER-GFP1–10
  • Species
    Synthetic
  • Insert Size (bp)
    750
  • Promoter UBQ10:sXVE:

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer cttcgcaagacccttcc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UBQ10:sXVE:ER-GFP1–10 was a gift from Tzu-Yin Liu (Addgene plasmid # 125664 ; http://n2t.net/addgene:125664 ; RRID:Addgene_125664)

  • For your References section:

    Using Tripartite Split-sfGFP for the Study of Membrane Protein-Protein Interactions. Liu TY. Methods Mol Biol. 2021;2200:323-336. doi: 10.1007/978-1-0716-0880-7_15. 10.1007/978-1-0716-0880-7_15 PubMed 33175385
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