pT3TS-HiFi-nCas9n-A72
(Plasmid #125646)

• Purpose: micro-injection of poly-adenylated HiFi-nCas9n RNA
• Depositing Lab: Jason Berman
• Publication: Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi: 10.1007/978-1-0716-0290-4_21.

Backbone

• Vector backbone: pT3TS
• Backbone size w/o insert (bp): 3310
• Total vector size (bp): 7490
• Modifications to backbone: polyA insert (72 As with restriction sites) was inserted
• Vector type: RNA in vitro transcription vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HiFi-nCas9n
• Alt name: HiFi-Cas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4179
• Mutation: R691A from wild-type Cas9
• Promoter: T3

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pT3TS-HiFi-nCas9n-pA.gb
• A portion of this plasmid was derived from a plasmid made by: Originally obtained from https://www.addgene.org/46757/, polyA insert was added and the HiFi R691A mutation was introduced.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry