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pT3TS-Hypa-nCas9n-A69
(Plasmid #125645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT3TS
  • Backbone size w/o insert (bp) 3306
  • Total vector size (bp) 7486
  • Modifications to backbone
    polyA insert (69 As with restriction sites) was inserted
  • Vector type
    RNA in vitro transcription vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hypa-nCas9n
  • Alt name
    Hypa-Cas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4179
  • Mutation
    N692A, M694A, Q695A, H698A from wild-type Cas9
  • Promoter T3

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Originally obtained from https://www.addgene.org/46757/, polyA insert was added and the Hypa-Cas9 mutations (N692A, M694A, Q695A, H698A) were introduced.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-Hypa-nCas9n-A69 was a gift from Jason Berman (Addgene plasmid # 125645 ; http://n2t.net/addgene:125645 ; RRID:Addgene_125645)

  • For your References section:

    Genome Editing in Zebrafish Using High-Fidelity Cas9 Nucleases: Choosing the Right Nuclease for the Task. Prykhozhij SV, Cordeiro-Santanach A, Caceres L, Berman JN. Methods Mol Biol. 2020;2115:385-405. doi: 10.1007/978-1-0716-0290-4_21. 10.1007/978-1-0716-0290-4_21 PubMed 32006412
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