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Addgene

1010Av2
(Plasmid #125642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125642 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyback
  • Backbone size w/o insert (bp) 6457
  • Total vector size (bp) 8849
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FAR protein
  • Insert Size (bp)
    615
  • Promoter 10xUAS

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTTTATATGAGACGAGAGTAAGGGGTC
  • 3′ sequencing primer CTTGACCTTGCCACAGAGGACTATT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1010Av2 was a gift from Omar Akbari (Addgene plasmid # 125642 ; http://n2t.net/addgene:125642 ; RRID:Addgene_125642)

  • For your References section:

    Parasitic nematode fatty acid- and retinol-binding proteins compromise host immunity by interfering with host lipid signaling pathways. Parks SC, Nguyen S, Nasrolahi S, Bhat C, Juncaj D, Lu D, Ramaswamy R, Dhillon H, Fujiwara H, Buchman A, Akbari OS, Yamanaka N, Boulanger MJ, Dillman AR. PLoS Pathog. 2021 Oct 29;17(10):e1010027. doi: 10.1371/journal.ppat.1010027. eCollection 2021 Oct. 10.1371/journal.ppat.1010027 PubMed 34714893
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