pcDNA 3.1(-) rat C/EBP beta (LIP)
(Plasmid #12562)

• Depositing Lab: Peter Johnson
• Publication: Johnson lab plasmids transferred from original pMEX to pcDNA (unpublished)

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCAAT/Enhancer-binding Protein beta LIP
• Alt name: C/EBP LIP
• Alt name: C/EBP beta
• Species: R. norvegicus (rat)
• Insert Size (bp): 438
• Entrez Gene: Cebpb (a.k.a. Il6dbp, NF-IL6, TCF5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). This plasmid is designed to express the LIP isoform of the protein.

For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).