pcDNA 3.1(-) mouse C/EBP beta (LIP)
(Plasmid #12561)

• Depositing Lab: Peter Johnson
• Publication: Johnson lab plasmids transferred from original pMEX to pcDNA (unpublished)

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CCAAT/Enhancer-binding Protein beta LIP
• Alt name: C/EBP beta LIP
• Alt name: C/EBP beta
• Species: M. musculus (mouse)
• Insert Size (bp): 461
• Entrez Gene: Cebpb (a.k.a. C/EBPbeta, CRP2, IL-6DBP, LAP, LIP, NF-IL6, NF-M, Nfil6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The C/EBPbeta gene encodes 3 in-frame translational isoforms (LAP*, LAP, and LIP). This plasmid is designed to express the LIP isoform of the protein.

For more information on the C/EBPbeta isoforms, please see the original reference:
Descombes, P., and Schibler, U. A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67:569-579 (1991).