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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 12560 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(-)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5427
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCCAAT enhancer binding protein gamma
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Alt nameC/EBP gamma
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SpeciesM. musculus (mouse)
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Insert Size (bp)477
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Entrez GeneCebpg (a.k.a. Gpe1bp, Ig/EBP, GPE1-BP, C/EBP[g], C/EBPgamma)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.1(-) mouse C/EBP gamma was a gift from Peter Johnson (Addgene plasmid # 12560 ; http://n2t.net/addgene:12560 ; RRID:Addgene_12560) -
For your References section:
Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP). Parkin SE, Baer M, Copeland TD, Schwartz RC, Johnson PF. J Biol Chem. 2002 Jun 28;277(26):23563-72. doi: 10.1074/jbc.M202184200. Epub 2002 Apr 29. 10.1074/jbc.M202184200 PubMed 11980905