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Addgene

pcDNA 3.1(-) mouse C/EBP gamma
(Plasmid #12560)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12560 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCAAT enhancer binding protein gamma
  • Alt name
    C/EBP gamma
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    477
  • Entrez Gene
    Cebpg (a.k.a. Gpe1bp, Ig/EBP, GPE1-BP, C/EBP[g], C/EBPgamma)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1(-) mouse C/EBP gamma was a gift from Peter Johnson (Addgene plasmid # 12560 ; http://n2t.net/addgene:12560 ; RRID:Addgene_12560)
  • For your References section:

    Regulation of CCAAT/enhancer-binding protein (C/EBP) activator proteins by heterodimerization with C/EBPgamma (Ig/EBP). Parkin SE, Baer M, Copeland TD, Schwartz RC, Johnson PF. J Biol Chem. 2002 Jun 28;277(26):23563-72. doi: 10.1074/jbc.M202184200. Epub 2002 Apr 29. 10.1074/jbc.M202184200 PubMed 11980905