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Addgene

pNIC28-BpWcbL
(Plasmid #125590)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125590 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNIC28
  • Backbone manufacturer
    SGC Oxford
  • Backbone size w/o insert (bp) 7284
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BpWcbL
  • Species
    Burkholderia pseudomallei
  • Insert Size (bp)
    1047
  • Entrez Gene
    wcbL (a.k.a. BPSL2796, gmhB)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7for
  • 3′ sequencing primer T7rev; gctagttattgctcagcgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNIC28-BpWcbL was a gift from Nicholas Harmer (Addgene plasmid # 125590 ; http://n2t.net/addgene:125590 ; RRID:Addgene_125590)

  • For your References section:

    Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery. Vivoli M, Isupov MN, Nicholas R, Hill A, Scott AE, Kosma P, Prior JL, Harmer NJ. Chem Biol. 2015 Dec 17;22(12):1622-32. doi: 10.1016/j.chembiol.2015.10.015. 10.1016/j.chembiol.2015.10.015 PubMed 26687481
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