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pcDNA 3.1(-) mouse C/EBP delta
(Plasmid #12559)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12559 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCAAT/Enhancer-binding Protein delta
  • Alt name
    C/EBP delta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    807
  • Mutation
    S2G introduced during cloning
  • Entrez Gene
    Cebpd (a.k.a. c/EBPdelta)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

When this C/EBPdelta construct was created, an NcoI restriction site was introduced that overlaps the start (AUG) codon to produce an optimal translation initiation site. This cloning strategy resulted in a S2G mutation in C/EBPdelta as a cloning "scar."

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1(-) mouse C/EBP delta was a gift from Peter Johnson (Addgene plasmid # 12559 ; http://n2t.net/addgene:12559 ; RRID:Addgene_12559)

  • For your References section:

    CAAT/enhancer-binding protein delta and cAMP-response element-binding protein mediate inducible expression of the nerve growth factor gene in the central nervous system. McCauslin CS, Heath V, Colangelo AM, Malik R, Lee S, Mallei A, Mocchetti I, Johnson PF. J Biol Chem. 2006 Jun 30;281(26):17681-8. doi: 10.1074/jbc.M600207200. Epub 2006 Apr 21. 10.1074/jbc.M600207200 PubMed 16632469
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