-
PurposeExpresses peroxisomal KillerRed (po-KR) in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 125585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP-N1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4001
- Total vector size (bp) 4683
-
Modifications to backboneRemoval of the BglII/NotI fragment
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsDepositing lab recommends using Top10F' cells
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepo-KillerRed
-
SpeciesSynthetic
-
Insert Size (bp)732
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pEGFP-N1-f
- 3′ sequencing primer EGFP-N1-R (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThe KillerRed template was obtained from Evrogen (pKillerRed-dmito plasmid)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMF1754 was a gift from Marc Fransen (Addgene plasmid # 125585 ; http://n2t.net/addgene:125585 ; RRID:Addgene_125585) -
For your References section:
Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk. Ivashchenko O, Van Veldhoven PP, Brees C, Ho YS, Terlecky SR, Fransen M. Mol Biol Cell. 2011 May;22(9):1440-51. doi: 10.1091/mbc.E10-11-0919. Epub 2011 Mar 3. 10.1091/mbc.E10-11-0919 PubMed 21372177