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Addgene

pMF1707
(Plasmid #125584)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125584 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4010
  • Total vector size (bp) 4733
  • Modifications to backbone
    Removal of the HindIII/NotI fragment
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab recommends using Top10F' cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-roGFP2
  • Species
    Synthetic
  • Insert Size (bp)
    723
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pEGFP-N1-f
  • 3′ sequencing primer pEGFP-N1-r
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Jim Remington (Institute of Molecular Biology, Oregon, USA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMF1707 was a gift from Marc Fransen (Addgene plasmid # 125584 ; http://n2t.net/addgene:125584 ; RRID:Addgene_125584)
  • For your References section:

    Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar cross-talk. Ivashchenko O, Van Veldhoven PP, Brees C, Ho YS, Terlecky SR, Fransen M. Mol Biol Cell. 2011 May;22(9):1440-51. doi: 10.1091/mbc.E10-11-0919. Epub 2011 Mar 3. 10.1091/mbc.E10-11-0919 PubMed 21372177