pMF1762
(Plasmid #125583)

• Purpose: Expresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cells
• Depositing Lab: Marc Fransen
• Publication: Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. doi: 10.1091/mbc.E10-11-0919. Epub 2011 Mar 3.

Backbone

• Vector backbone: pKillerRed-dmito
• Backbone manufacturer: Evrogen
• Backbone size w/o insert (bp): 4129
• Total vector size (bp): 4852
• Modifications to backbone: removal of the BamHI/NotI fragment
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Depositing lab recommends using Top10F' cells
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mt-roGFP2
• Species: Synthetic
• Insert Size (bp): 924
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pEGFP-N1-f
• 3′ sequencing primer: pEGFP-C-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Jim Remington (Institute of Molecular Biology, Oregon, USA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry