pCAFNF-PSDd1.2-GFP
(Plasmid #125581)

• Purpose: Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.
• Depositing Lab: Takeshi Imai
• Publication: Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e7. doi: 10.1016/j.devcel.2023.05.004. Epub 2023 Jun 7.

Backbone

• Vector backbone: pCAFNF
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSDd1.2-GFP
• Alt name: PSD95 deletion mutant
• Alt name: Dlg4
• Species: M. musculus (mouse)
• Entrez Gene: Dlg4 (a.k.a. Dlgh4, PSD-95, PSD95, SAP90, SAP90A)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • 125581 pCAFNF-PSDd1_2-GFP.gb
• A portion of this plasmid was derived from a plasmid made by: 13385pCALNL vector is derived from Addgene #13770. EGFP is from Clontech.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PSDd1.2-GFP was described in PMID: 10402201.
Arnold, D. B. & Clapham, D. E.Molecular determinants for subcellular localizationof PSD-95 with an interacting K1 channel. Neuron 23, 149–157 (1999).

Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.