pCALNL-NaChBac
(Plasmid
#125580)
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PurposeCre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCALNL
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Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNaChBac
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SpeciesBacillus Hallodurans C-125
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypCALNL vector is derived from Addgene #13770. NaChBac is derived from Clapham lab (Harvard).
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/625616v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCALNL-NaChBac was a gift from Takeshi Imai (Addgene plasmid # 125580 ; http://n2t.net/addgene:125580 ; RRID:Addgene_125580) -
For your References section:
Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Dev Cell. 2023 Jul 24;58(14):1221-1236.e7. doi: 10.1016/j.devcel.2023.05.004. Epub 2023 Jun 7. 10.1016/j.devcel.2023.05.004 PubMed 37290446