hRad51(K133R)
(Plasmid
#125571)
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PurposeExpresses the hRad51(K133R) protein in mammalian cells under CMV promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 125571 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCMV
- Backbone size w/o insert (bp) 3300
- Total vector size (bp) 4422
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRad51
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SpeciesH. sapiens (human)
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MutationK133R
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Entrez GeneRAD51 (a.k.a. BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hRad51(K133R) was a gift from David Liu (Addgene plasmid # 125571 ; http://n2t.net/addgene:125571 ; RRID:Addgene_125571) -
For your References section:
Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks. Rees HA, Yeh WH, Liu DR. Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4. 10.1038/s41467-019-09983-4 PubMed 31101808