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Addgene

hRad51(A89E)–Cas9(D10A)
(Plasmid #125563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Backbone size w/o insert (bp) 3300
  • Total vector size (bp) 8712
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rad51(A89E)–Cas9(D10A)
  • Alt name
    RDN
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAD51 (a.k.a. BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hRad51(A89E)–Cas9(D10A) was a gift from David Liu (Addgene plasmid # 125563 ; http://n2t.net/addgene:125563 ; RRID:Addgene_125563)

  • For your References section:

    Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks. Rees HA, Yeh WH, Liu DR. Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4. 10.1038/s41467-019-09983-4 PubMed 31101808
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