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Addgene

HI.HslU.RSETB.fl.wt
(Plasmid #12556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12556 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET_B
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Haemophilus influenzae heat shock locus U
  • Alt name
    H. influenzae HslU
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    1400
  • Mutation
    none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original insert cloned from two plasmids of the H. influenzae genomic sequencing project obtained from ATCC

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This expression plasmid was constructed from expression plasmid for HslUV complex by deleting coding sequence for HslV and inter-gene spacer

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HI.HslU.RSETB.fl.wt was a gift from David McKay (Addgene plasmid # 12556 ; http://n2t.net/addgene:12556 ; RRID:Addgene_12556)

  • For your References section:

    Structure of Haemophilus influenzae HslU protein in crystals with one-dimensional disorder twinning. Trame CB, McKay DB. Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1079-90. 10.1107/S0907444901007673 PubMed 11468391
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