nesprin TSmod 25
(Plasmid #125555)

• Purpose: Nesprin-2G force sensor with cerulean-F25-venus TSmodule
• Depositing Lab: Daniel Conway
• Publication: Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-300. doi: 10.1007/s12195-019-00579-y. eCollection 2019 Aug.

Backbone

• Vector backbone: pcdna 3.1
• Backbone manufacturer: Invitroogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 9400
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mini-nesprin-2G
• Alt name: SYNE2
• Species: M. musculus (mouse)
• Insert Size (bp): 4000
• Mutation: TSmod changed from mTFP to cerulean, flageliform linker shorted from 40 amino acids to 25
• Entrez Gene: Syne2 (a.k.a. 6820443O06Rik, Cpfl8, D12Ertd777e, KASH2, NUA, Nesp2g, dice, mKIAA1011)
• Promoter: CMV
• Tag / Fusion Protein:
  • cerulean, venus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry