mcherry-DN KASH
(Plasmid #125553)

• Purpose: mcherry labeled DN-KASH
• Depositing Lab: Daniel Conway
• Publication: Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-300. doi: 10.1007/s12195-019-00579-y. eCollection 2019 Aug.

Backbone

• Vector backbone: pmcherry N1
• Backbone manufacturer: clonetech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 4900
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SYNE1
• Alt name: DN-KASH
• Species: H. sapiens (human)
• Insert Size (bp): 216
• Mutation: Truncated form: only the KASH domain of nesprin-1 is expressed to create a dominant negative
• GenBank ID: NM_182961.4
• Entrez Gene: SYNE1 (a.k.a. 8B, AMC3, AMCM, ARCA1, C6orf98, CPG2, EDMD4, KASH1, MYNE1, Nesp1, SCAR8, dJ45H2.2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mcherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The KASH-1 sequence was synthetically produced by GeneArt.
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry