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Addgene

pDEST-N2H-C1
(Plasmid #125549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 125549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom made
  • Backbone manufacturer
    we built
  • Backbone size (bp) 10101
  • Vector type
    Mammalian Expression, Yeast Expression ; in vitro expression
  • Promoter synthetic promoter containing yeast, mammalian, and in vitro expression elements
  • Selectable markers
    LEU2
  • Tag / Fusion Protein
    • NanoLuc fragment1 attached to c-terminus of Gateway cloning site (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results identified an IS4-like element located between the Chloramphenicol resistance and ccdB genes. This element will not affect plasmid function. Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-N2H-C1 was a gift from Marc Vidal (Addgene plasmid # 125549 ; http://n2t.net/addgene:125549 ; RRID:Addgene_125549)

  • For your References section:

    Maximizing binary interactome mapping with a minimal number of assays. Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal M, Calderwood MA, Jacob Y. Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2. 10.1038/s41467-019-11809-2 PubMed 31467278
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