pQE60-Exp5
(Plasmid #12553)

• Depositing Lab: Ian Macara
• Publication: Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Backbone

• Vector backbone: pQE60
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EXPORTIN-5
• Alt name: XPO5
• Alt name: EXP5
• Species: H. sapiens (human)
• Insert Size (bp): 3600
• Mutation: NONE
• GenBank ID: NM_020750
• Entrez Gene: XPO5 (a.k.a. exp5)
• Tag / Fusion Protein:
  • HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: pQE

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For expression of this protein, supplement medium with2% ethanol; induce with 300 uM IPTG; add 25 mM beta-mercaptoethanol to the lysis buffer. Maintain 7 mM mercaptoethanol in all buffers for purification and storage.