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Addgene

pKmyc-Exp5
(Plasmid #12552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12552 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKmyc
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Exportin-5
  • Alt name
    EXP5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3600
  • Mutation
    None
  • GenBank ID
    NM_020750
  • Entrez Gene
    XPO5 (a.k.a. exp5)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (destroyed during cloning)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer CAGGTCCAACTGCACCTCGGTTC
  • 3′ sequencing primer TTTGTGATGCTATTGCTTTATTTGTA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKmyc-Exp5 was a gift from Ian Macara (Addgene plasmid # 12552 ; http://n2t.net/addgene:12552 ; RRID:Addgene_12552)

  • For your References section:

    Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins. Brownawell AM, Macara IG. J Cell Biol. 2002 Jan 7. 156(1):53-64. 10.1083/jcb.200110082 PubMed 11777942
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