Skip to main content
Addgene

pcDNA3.1(-) rat C/EBP alpha
(Plasmid #12550)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12550 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCAAT/Enhancer-binding Protein alpha
  • Alt name
    C/EBP alpha
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1077
  • Entrez Gene
    Cebpa (a.k.a. DBPCEP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-) rat C/EBP alpha was a gift from Peter Johnson (Addgene plasmid # 12550 ; http://n2t.net/addgene:12550 ; RRID:Addgene_12550)

  • For your References section:

    Structural basis for DNA recognition by the basic region leucine zipper transcription factor CCAAT/enhancer-binding protein alpha. Miller M, Shuman JD, Sebastian T, Dauter Z, Johnson PF. J Biol Chem. 2003 Apr 25;278(17):15178-84. doi: 10.1074/jbc.M300417200. Epub 2003 Feb 10. 10.1074/jbc.M300417200 PubMed 12578822
Related items:
Commonly requested with: